rabbit anti human igfbp2 polyclonal antibody  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Epidermal loss of PRMT5 leads to the emergence of an atypical basal keratinocyte-like cell population and defective skin stratification

doi: 10.1101/2024.11.08.620904

Figure Lengend Snippet: ( A-M ) scRNA-seq violin plots for Cdkn1a (A), Igfbp2 (E), Trp63 (H), and Krt17 (K) in the novel (19) and basal (3/5) keratinocyte clusters. Corresponding α-CDKN1A (B-C), α-IGFBP2 (F-G), α-TRP63 (I-J), and α-KRT17 (L-M) immunofluorescence from E15.5 (B, C, F, G, I, J) or E17.5 (L, M) CTRL (B, F, I, L) or Prmt5 cKO (C, G, J, M) embryos. Integrative Genomics Viewer (IGV) reveals a suggestive (FC=1.74, p_ adj=0.51) locus specific increase in chromatin accessibility at the Cdkn1a promoter (D). ( N ) A schematic summarizing the normal progression of keratinocytes in CTRL (top) and atypical progression in Prmt5 cKOs (bottom), including the molecular features involved. Sections shown are the craniofacial epidermis, lateral to the oral cavity, in a coronal plane. White dashed lines demarcate the boundary of the dermis and epidermis. Nuclei are counterstained with DAPI. All section immunofluorescence images are Z-stacks compiled with ‘Max Intensity’ in FIJI. Scale bar = 10 µm.

Article Snippet: Primary antibodies include: α-CDKN1A (1:50, ProteinTech, 10355-1-AP), α-CLDN1 (1:60, Abcam, ab15098), α-COL4 (1:500, Invitrogen, PA1-85320), α-eGFP (1:500, Novus Biologicals, NB600-308), α-FLG (1:500, Biolegend, 905804), α-IGFBP2 (1:200, Bioss, bs-1108R), α-KI67 (1:200, Abcam, ab16667), α-KRT6 (1:500, Biolegend, PRB-169F), α-KRT10 (1:500, Biolegend, 905404), α-KRT14 (1:20, SantaCruz, sc-53253), α-KRT17 (1:1000, Proteintech, 17516-1-AP), α-NF (0.5ug/ml, Hybridoma, AB531793), α-PRMT5 (1:200, Millipore, 07-405), α-TRP63 (1:500, Cell Signaling, 13109S), and α-VCL (1:300, Sigma, V9131).

Techniques: Immunofluorescence

Journal: bioRxiv

Article Title: Epidermal loss of PRMT5 leads to the emergence of an atypical basal keratinocyte-like cell population and defective skin stratification

doi: 10.1101/2024.11.08.620904

Figure Lengend Snippet: ( A-C ) IGV views of Igfbp2 , Krt17 , and Trp63 , corresponding to their expression changes in . ( D ) Heatmap of select genes (rows) and their average expression level (red = high, blue = low) in distinct epidermal clusters (rows). Each cluster of genes is sectioned into their corresponding broad category.

Article Snippet: Primary antibodies include: α-CDKN1A (1:50, ProteinTech, 10355-1-AP), α-CLDN1 (1:60, Abcam, ab15098), α-COL4 (1:500, Invitrogen, PA1-85320), α-eGFP (1:500, Novus Biologicals, NB600-308), α-FLG (1:500, Biolegend, 905804), α-IGFBP2 (1:200, Bioss, bs-1108R), α-KI67 (1:200, Abcam, ab16667), α-KRT6 (1:500, Biolegend, PRB-169F), α-KRT10 (1:500, Biolegend, 905404), α-KRT14 (1:20, SantaCruz, sc-53253), α-KRT17 (1:1000, Proteintech, 17516-1-AP), α-NF (0.5ug/ml, Hybridoma, AB531793), α-PRMT5 (1:200, Millipore, 07-405), α-TRP63 (1:500, Cell Signaling, 13109S), and α-VCL (1:300, Sigma, V9131).

Techniques: Expressing